Faster sequence alignment through GPU-accelerated restriction of the seed-and-extend search space

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Abstract

Motivation

In computing pairwise alignments of biological sequences, software implementations employ a variety of heuristics that decrease the computational effort involved in computing potential alignments. A key element in achieving high processing throughput is to identify and prioritize potential alignments where high-scoring mappings can be expected. These tasks involve listprocessing operations that can be efficiently performed on GPU hardware.

Results

We implemented a read aligner called A21 that exploits GPU-based parallel sort and reduction techniques to restrict the number of locations where potential alignments may be found. When compared with other high-throughput aligners, this approach finds more high-scoring mappings without sacrificing speed or accuracy. A21 running on a single GPU is about 10 times faster than comparable CPU-based tools; it is also faster and more sensitive in comparison with other recent GPU-based aligners.

Availability

The A21 software is open source and available at<ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="https://github.com/RWilton/A21">https://github.com/RWilton/A21</ext-link>.

Contact

<email>rwilton@pha.jhu.edu</email>

Supplementary information

Supplementary results are available at <<<TBD>>>

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