NanoSim: nanopore sequence read simulator based on statistical characterization

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Abstract

Motivation:

In 2014, Oxford Nanopore Technologies (ONT) announced a new sequencing platform called MinION. The particular features of MinION reads – longer read lengths and single-molecule sequencing in particular – show potential for genome characterization. As of yet, the pre-commercial technology is exclusively available through early-access, and only a few datasets are publically available for testing. Further, no software exists that simulates MinION platform reads with genuine ONT characteristics.

Results:

In this article, we introduce NanoSim, a fast and scalable read simulator that captures the technology-specific features of ONT data, and allows for adjustments upon improvement of nanopore sequencing technology.

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