Arabidopsis phenotyping through Geometric Morphometrics

In recent years, much technical progress has been done regarding plant phenotyping including the model speciesArabidopsis thaliana. With automated, high-throughput platforms and the development of improved algorithms for the rosette segmentation task, it is now possible to massively extract reliable shape and size parameters for genetic, physiological and environmental studies. The development of low-cost phenotyping platforms and freeware resources make it possible to widely expand phenotypic analysis tools for Arabidopsis. However, objective descriptors of shape parameters that could be used independently of platform and segmentation software used are still lacking and shape descriptions still rely onad hocor even sometimes contradictory descriptors, which could make comparisons difficult and perhaps inaccurate. Modern geometric morphometrics is a family of methods in quantitative biology proposed to be the main source of data and analytical tools in the emerging field of phenomics studies. It has been used for taxonomists and paleontologists for decades and is now a mature discipline. By combining geometry, multivariate analysis and powerful statistical techniques, it offers the possibility to reproducibly and accurately account for shape variations amongst groups. Based on the location of homologous landmarks points over photographed or scanned specimens, these tools could identify the existence and degree of shape variation and measure them in standard units. Here, it is proposed a particular scheme of landmarks placement on Arabidopsis rosette images to study shape variation in the case study of viral infection processes. Several freeware-based geometric morphometric tools are applied in order to exemplify the usefulness of this approach to the study of phenotypes in this model plant. These methods are concisely presented and explained. Shape differences between controls and infected plants are quantified throughout the infectious process and visualized with the appealing graphs that are a hallmark of these techniques and render complex mathematical analysis simple outcomes to interpret. Quantitative comparisons between two unrelated ssRNA+ viruses are shown and reproducibility issues are assessed. Combined with the newest automatons and plant segmentation procedures, geometric morphometric tools could boost phenotypic features extraction and processing in an objective, reproducible manner.