Ad hoc laboratory-based surveillance of SARS-CoV-2 by real-time RT-PCR using minipools of RNA prepared from routine respiratory samples

  1. Anna M. Eis-Hübinger
  2. Mario Hönemann
  3. Jürgen J. Wenzel
  4. Annemarie Berger
  5. Marek Widra
  6. Barbara Schmidt
  7. Souhaib Aldabbagh
  8. Benjamin Marx
  9. Hendrik Streeck
  10. Sandra Ciesek
  11. Uwe. G. Liebert
  12. Daniela Huzly
  13. Hartmut Hengel
  14. Marcus Panning
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Abstract

We report a laboratory-based surveillance for SARS-CoV-2 using minipools of respiratory samples submitted for routine diagnostics. We tested a total of 70 minipools resembling 700 samples shortly before the upsurge of cases in Germany. We identified one SARS-CoV-2 positive patient. Our approach proved its concept, is easily adaptable and resource-saving.

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