A 5-min RNA preparation method for COVID-19 detection with RT-qPCR

  1. Alim Ladha
  2. Julia Joung
  3. Omar O. Abudayyeh
  4. Jonathan S. Gootenberg
  5. Feng Zhang
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Abstract

RNA extraction has become a bottleneck for detection of COVID-19, in part because of reagent shortages. We present here a rapid protocol that circumvents the need for RNA extraction that is compatible with RT-qPCR-based detection methods.

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