Rational Design of Minimal Synthetic Promoters for Plants

Promoters serve a critical role in establishing baseline transcriptional capacity through the recruitment of proteins, including transcription factors (TFs). Previously, a paucity of data forcis-regulatory elements in plants meant that it was challenging to determine which sequence elements in plant promoter sequences contributed to transcriptional function. In this study, we have identified functional elements in the promoters of plant genes and plant pathogens that utilise plant transcriptional machinery for gene expression. We have established a quantitative experimental system to investigate transcriptional function, investigating how identity, density and position contribute to regulatory function. We then identified permissive architectures for minimal synthetic plant promoters enabling computational design of a suite of synthetic promoters of different strengths. These have been used to regulate the relative expression of output genes in simple genetic devices.<fig id="ufig1" position="float" fig-type="figure" orientation="portrait"><graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="095406v1_ufig1" position="float" orientation="portrait"/></fig>