Neutralization Assay with SARS-CoV-1 and SARS-CoV-2 Spike Pseudotyped Murine Leukemia Virions

  1. Yue Zheng
  2. Erin T. Larragoite
  3. Juan Lama
  4. Isabel Cisneros
  5. Julio C. Delgado
  6. Patricia Slev
  7. Jenna Rychert
  8. Emily A. Innis
  9. Elizabeth S.C.P. Williams
  10. Mayte Coiras
  11. Matthew T. Rondina
  12. Adam M. Spivak
  13. Vicente Planelles
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Abstract

Antibody neutralization is an important prognostic factor in many viral diseases. To easily and rapidly measure titers of neutralizing antibodies in serum or plasma, we developed pseudovirion particles composed of the spike glycoprotein of SARS-CoV-2 incorporated onto murine leukemia virus capsids and a modified minimal MLV genome encoding firefly luciferase. These pseudovirions provide a practical means of assessing immune responses under laboratory conditions consistent with biocontainment level 2.

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