Acrylamide Fragment Inhibitors that Induce Unprecedented Conformational Distortions in Enterovirus 71 3C and SARS-CoV-2 Main Protease

RNA viruses are critically dependent upon virally encoded proteases that cleave the viral polyproteins into functional mature proteins. Many of these proteases are structurally conserved with an essential catalytic cysteine and this offers the opportunity to irreversibly inhibit these enzymes with electrophilic small molecules. Here we describe the successful application of quantitative irreversible tethering (qIT) to identify acrylamide fragments that selectively target the active site cysteine of the 3C protease (3C^pro) of Enterovirus 71, the causative agent of hand, foot and mouth disease in humans, altering the substrate binding region. Further, we effectively re-purpose these hits towards the main protease (M^pro) of SARS-CoV-2 which shares the 3C-like fold as well as similar catalytic-triad. We demonstrate that the hit fragments covalently link to the catalytic cysteine of M^proto inhibit its activity. In addition, we provide the first demonstration that targeting the active site cysteine of M^procan also have profound allosteric effects, distorting secondary structures required for formation of the active dimeric unit of M^pro. These new data provide novel mechanistic insights into the design of EV71 3C^proand SARS-CoV-2 M^proinhibitors and identify acrylamide-tagged pharmacophores for elaboration into more selective agents of therapeutic potential.