Sub-5-minute Detection of SARS-CoV-2 RNA using a Reverse Transcriptase-Free Exponential Amplification Reaction, RTF-EXPAR

  1. Jake G. Carter
  2. Lorea Orueta Iturbe
  3. Jean-Louis H. A. Duprey
  4. Ian R. Carter
  5. Craig D. Southern
  6. Marium Rana
  7. Andrew Bosworth
  8. Andrew D. Beggs
  9. Matthew R. Hicks
  10. James H. R. Tucker
  11. Timothy R. Dafforn
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Abstract

We report a rapid isothermal method for detecting SARS-CoV-2, the virus responsible for COVID-19. The procedure uses a novel reverse transcriptase-free (RTF) approach for converting RNA into DNA, which triggers a rapid amplification using the Exponential Amplification Reaction (EXPAR). Deploying the RNA-to-DNA conversion and amplification stages of the RTF-EXPAR assay in a single step results in the detection of a sample of patient SARS-CoV-2 RNA in under 5 minutes.

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