Detection and stability of SARS-CoV-2 fragments in wastewater: Impact of storage temperature

  1. Rudolf Markt
  2. Markus Mayr
  3. Evelyn Peer
  4. Andreas O. Wagner
  5. Nina Lackner
  6. Heribert Insam
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Abstract

SARS-CoV-2 wastewater epidemiology suffers from uncertainties concerning sample storage. We show the effect of storage of wastewater on the detectable SARS-CoV-2 load. Storage at 4 °C up to 9 days had no significant effect, while storage at −20 °C led to a significant reduction in gene copy numbers.

Highlights

  • Raw wastewater samples can be stored up to 9 days at 4°C for SARS-CoV-2 wastewater surveillance

  • Freezing of wastewater sample dramatically decreases RT-qPCR signal of SARS-CoV-2 from wastewater.

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