A rapid assessment of wastewater for genomic surveillance of SARS-CoV-2 variants at sewershed scale in Louisville, KY

  1. J. L. Fuqua
  2. E.C. Rouchka
  3. S. Waigel
  4. K. Sokoloski
  5. D. Chung
  6. W. Zacharias
  7. M. Zhang
  8. J. Chariker
  9. D. Talley
  10. I. Santisteban
  11. A. Varsani
  12. S. Moyer
  13. R. H. Holm
  14. R. A. Yeager
  15. T. Smith
  16. A. Bhatnagar
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Abstract

In this communication, we report on the genomic surveillance of SARS-CoV-2 using wastewater samples in Jefferson County, KY. In February 2021, we analyzed seven wastewater samples for SARS-CoV-2 genomic surveillance. Variants observed in smaller catchment areas, such as neighborhood manhole locations, were not necessarily consistent when compared to associated variant results in downstream treatment plants, suggesting catchment size or population could impact the ability to detect diversity.

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