Implementation of a qPCR assay coupled with genomic surveillance for real-time monitoring of SARS-CoV-2 variants of concern

  1. Natalia Rego
  2. Alicia Costábile
  3. Mercedes Paz
  4. Cecilia Salazar
  5. Paula Perbolianachis
  6. LucĂ­a Spangenberg
  7. Ignacio Ferrés
  8. Rodrigo Arce
  9. Alvaro Fajardo
  10. Mailen Arleo
  11. Tania Possi
  12. Inés Bellini
  13. Lucia Bilbao
  14. Natalia Reyes
  15. Ma Noel Bentancor
  16. Andrés Lizasoain
  17. María José Benítez
  18. MatĂ­as Castells
  19. MatĂ­as Victoria
  20. Leticia Maya
  21. Viviana Bortagaray
  22. Ana Moller
  23. Gonzalo Bello
  24. Ighor Arantes
  25. Mariana Brandes
  26. Pablo Smircich
  27. Odhille Chappos
  28. Melissa DuquĂ­a
  29. Belén González
  30. Luciana Griffero
  31. Mauricio MĂ©ndez
  32. Ma PĂ­a Techera
  33. Juan Zanetti
  34. Bernardina Rivera
  35. MatĂ­as Maidana
  36. Martina Alonso
  37. Cecilia Alonso
  38. Julio Medina
  39. Henry Albornoz
  40. Rodney Colina
  41. Veronica Noya
  42. Gregorio Iraola
  43. Tamara Fernández-Calero
  44. Gonzalo Moratorio
  45. Pilar Moreno
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Abstract

We developed a genomic surveillance program for real-time monitoring of SARS-CoV-2 variants of concern in Uruguay. Here, we present the first results, including the proposed qPCR-VOC method, the general workflow and the report of the introduction and community transmission of the VOC P.1 in Uruguay in multiple independent events.

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