Glutamine synthetase mRNA releases sRNA from its 3’UTR to regulate carbon/nitrogen metabolic balance

Glutamine synthetase is the key enzyme of nitrogen assimilation, which is encoded in the first cistron ofglnALGoperon and is induced under nitrogen limiting conditions through transcriptional activation by NtrBC inSalmonellaandE. coli. 2-oxoglutarate serves as the carbon skeleton of glutamate and glutamine, but how 2-oxoglutarate fluctuation is controlled in response to nitrogen availability remained unknown. We show that theglnAmRNA produces an Hfq-dependent GlnZ sRNA from its 3’ sUTR through RNase E-mediated cleavage. Through a base-pairing mechanism, GlnZ primarily regulatessucA, encoding the E1o component of 2-oxoglutarate dehydrogenase. In the cells grown on glutamine as the nitrogen source, the endogenous GlnZ represses the expression of SucA to redirect the carbon flow from the TCA cycle to the nitrogen assimilation pathway. This study also clarifies that the release of GlnZ sRNA from theglnAmRNA by RNase E is essential for the post-transcriptional regulation ofsucA, and thus the mRNA coordinates the two independent functions to balance the supply and demand of the fundamental metabolites.