AlphaPulldown – a Python package for protein-protein interaction screens using AlphaFold-Multimer

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Abstract

Summary

The Artificial Intelligence-based structure prediction program AlphaFold-Multimer enabled structural modelling of protein complexes with unprecedented accuracy. Increasingly, AlphaFold-Multimer is also used to discover new protein-protein interactions. Here, we present AlphaPulldown, a Python package that streamlines protein-protein interaction screens and high-throughput modelling of higher-order oligomers using AlphaFold-Multimer. It provides a convenient command line interface, a variety of confidence scores, and a graphical analysis tool.

Availability and implementation

AlphaPulldown is freely available at <ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="https://www.embl-hamburg.de/AlphaPulldown">https://www.embl-hamburg.de/AlphaPulldown</ext-link>.

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