ChIP-MS reveals the local chromatin composition by label-free quantitative proteomics

  1. Wai Khang Yong
  2. Grishma Rane
  3. Nurkaiyisah Zaal Anuar
  4. Xiaoman Shao
  5. Chai Yeen Goh
  6. Vartika Khanchandani
  7. Vivian L. S. Kuan
  8. Maya Jeitany
  9. H. Phillip Koeffler
  10. Lih-Wen Deng
  11. Takaomi Sanda
  12. Dennis Kappei
4 evaluations Published on
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Abstract

Chromatin immunoprecipitation (ChIP) has been a cornerstone for epigenetic analyses over the last decades, but even coupled to sequencing approaches (ChIP-seq), it is ultimately limited to one protein at a time. In a complementary effort, we here combined ChIP with label-free quantitative (LFQ) mass spectrometry (ChIP-MS) to interrogate local chromatin compositions. We demonstrate the versatility of our approach at telomeres, with transcription factors, in tissue and by dCas9-driven locus-specific enrichment.

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