Ly6G⁺Granulocytes-derived IL-17 limits protective host responses and promotes tuberculosis pathogenesis

The protective correlates ofMycobacterium tuberculosis(Mtb) infection-elicited host immune responses are incompletely understood. Here, we report pro-pathogenic crosstalk involving Ly6G⁺granulocytes (Ly6G⁺Gra), IL-17 and COX2. We show that in the lungs ofMtb-infected wildtype mice, either BCG-vaccinated or not, most intracellular bacilli are Ly6G⁺Gra-resident four weeks post-infection onwards. In the genetically susceptibleIFNγ^−/−mice, excessive Ly6G⁺Gra infiltration correlates with severe bacteraemia. Neutralizing IL-17 (anti-IL17mAb) and COX2 inhibition by celecoxib reverse Ly6G⁺Gra infiltration, associated pathology and death inIFNγ^−/−mice. Surprisingly, Ly6G⁺Gra also serves as the major source of IL-17 in the lungs ofMtb-infected WT orIFNγ^−/−mice. The IL-17-COX2-Ly6G⁺Gra interplay also operates in WT mice. Inhibiting RORγt, the key transcription factor for IL-17 production or COX2, reduces the bacterial burden in Ly6G⁺Gra, leading to reduced bacterial burden and pathology in the lungs of WT mice. In theMtb-infected WT mice, COX2 inhibition abrogates IL-17 levels in the lung homogenates and significantly enhances BCG’s protective efficacy, mainly by targeting the Ly6G⁺Gra-residentMtbpool. Furthermore, in pulmonary TB patients, high neutrophil count and IL-17 correlated with adverse treatment outcomes. Together, our results suggest that IL-17 and PGE2 are the negative correlates of protection, and we propose targeting the pro-pathogenic IL-17-COX2-Ly6G⁺Gra axis for TB prevention and therapy.