Genome assembly of the milky mangrove Excoecaria agallocha

  1. Hong Kong Biodiversity Genomics Consortium
  2. Project Coordinator and Co-Principal Investigators
  3. Jerome H.L. Hui
  4. Ting Fung Chan
  5. Leo L. Chan
  6. Siu Gin Cheung
  7. Chi Chiu Cheang
  8. James K.H. Fang
  9. Juan Diego Gaitan-Espitia
  10. Stanley C.K. Lau
  11. Yik Hei Sung
  12. Chris K.C. Wong
  13. Kevin Y.L. Yip
  14. Yingying Wei
  15. DNA extraction, library preparation and sequencing
  16. Sean T.S. Law
  17. Wai Lok So
  18. Genome assembly and gene model prediction
  19. Wenyan Nong
  20. Genome analysis and quality control
  21. Wenyan Nong
  22. Sample collector and logistics
  23. David T.W. Lau
  24. Sean T.S. Law
  25. Shing Yip Lee
  26. Ho Yin Yip
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Abstract

The milky mangrove Excoecaria agallocha is a latex-secreting mangrove that are distributed in tropical and subtropical regions. While its poisonous latex is regarded as a potential source of phytochemicals for biomedical applications, the genomic resources of E. agallocha remains limited. Here, we present a chromosomal level genome of E. agallocha , assembled from the combination of PacBio long-read sequencing and Omni-C data. The resulting assembly size is 1,332.45 Mb and has high contiguity and completeness with a scaffold N50 of 58.9 Mb and a BUSCO score of 98.4 %. 73,740 protein-coding genes were also predicted. The milky mangrove genome provides a useful resource for further understanding the biosynthesis of phytochemical compounds in E. agallocha .

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