Autoprot: Processing, Analysis and Visualization of Proteomics Data in Python

The increasing numbers of complex quantitative mass spectrometry-based proteomics data sets demand a standardised and reliable analysis pipeline. For this purpose, Python-based analysis, particularly through Jupyter notebooks, serves as a simple yet powerful tool. Nevertheless, the availability of Python software for standardised and accessible MS data analysis is limited, and this software is often constrained to using analysis functions written in Python. This excludes existing and well-tested software, for example written in R. Despite this, Python offers several interactive data visualisation modules that greatly enhance exploratory research and facilitate result communication with collaboration partners. Consequently, there is a need for an integrated and Jupyter-compatible Python analysis pipeline that incorporates R algorithms and interactive visualization for proteomics data analysis.