Ca²⁺binding to Esyt is required to modulate membrane contact site density inDrosophilaphotoreceptors

Membrane Contact Sites (MCS) between the plasma membrane (PM) and endoplasmic reticulum (ER) have been shown to regulate Ca²⁺influx into animal cells. However, the mechanisms by which cells modulate ER-PM MCS density is not understood and the role of Ca²⁺, if any, in regulating this process is not known. We report that inDrosophilaphotoreceptors, MCS density is dependent on the activity of the Ca²⁺permeable channels-TRP and TRPL. This regulation of MCS density by Ca²⁺is mediated by extended synaptotagmin (dEsyt), a protein localised to ER-PM MCS in photoreceptors and previously shown to regulate MCS density. We find that the Ca²⁺binding activity of dEsyt is required for its functional activityin vivo. dEsyt^CaBM, a Ca²⁺non-binding mutant of dEsyt is unable to modulate MCS structure in a manner equivalent to its wild type counterpart. Further, when reconstituted in dEsyt null photoreceptors, in contrast to wild type dEsyt, dEsyt^CaBMis unable to rescue ER-PM MCS density and other key phenotypes. Finally, when expressed in wild type photoreceptors, dEsyt^CaBMphenocopies loss of dEsyt function. Taken together, our data supports a role for the Ca²⁺binding activity of dEsyt in regulating the ER-PM MCS density in photoreceptors thus tuning signal transduction in response to Ca²⁺influx triggered by ambient illumination.