TRPγ regulates lipid metabolism through Dh44 neuroendocrine cells

Understanding how the brain controls nutrient storage is pivotal. Transient receptor potential (TRP) channels are conserved from insects to humans. They serve in detecting environmental shifts and in acting as internal sensors. Here, we found that a TRPγ mutant exhibited inDrosophila melanogasterare required for maintaining normal lipid and protein levels. In animals, lipogenesis and lipolysis control lipid levels in response to food availability. Lipids are mostly stored as triacylglycerol in the fat bodies (FBs) ofD. melanogaster. Interestingly,trpγdeficient mutants exhibited elevated TAG levels and our genetic data indicated thatDh44neurons are indispensable for normal lipid storage but not protein storage. Thetrpγmutants also exhibited reduced starvation resistance, which was attributed to insufficient lipolysis in the FBs. This could be mitigated by administering lipase or metformin orally, indicating a potential treatment pathway. Gene expression analysis indicated thattrpγknockout downregulatedbrummer, a key lipolytic gene, resulting in chronic lipolytic deficits in the gut and other fat tissues. The study also highlighted the role of specific proteins, including neuropeptide DH44 and its receptor DH44R2 in lipid regulation. Our findings provide insight into the broader question of how the brain regulates nutrient storage.