Unveiling Vertebrate Development Dynamics in FrogXenopus laevisusing Micro-CT Imaging
Abstract
Background
Xenopus laevis, the African clawed frog, is a versatile vertebrate model organism employed across various biological disciplines, prominently in developmental biology to elucidate the intricate processes underpinning body plan reorganization during metamorphosis. Despite its widespread utility, a notable gap exists in the availability of comprehensive datasets encompassingXenopus’late developmental stages.
Findings
In the present study, we harnessed micro-computed tomography (micro-CT), a non-invasive 3D imaging technique utilizing X-rays to examine structures at a micrometer scale, to investigate the developmental dynamics and morphological changes of this crucial vertebrate model. Our approach involved generating high-resolution images and computed 3D models of developingXenopusspecimens, spanning from premetamorphosis tadpoles to fully mature adult frogs. This extensive dataset enhances our understanding of vertebrate development and is adaptable for various analyses. For instance, we conducted a thorough examination, analyzing body size, shape, and morphological features, with a specific emphasis on skeletogenesis, teeth, and organs like the brain at different stages. Our analysis yielded valuable insights into the morphological changes and structure dynamics in 3D space duringXenopus’development, some of which were not previously documented in such meticulous detail. This implies that our datasets effectively capture and thoroughly examineXenopusspecimens. Thus, these datasets hold the solid potential for additional morphological and morphometric analyses, including individual segmentation of both hard and soft tissue elements withinXenopus.
Conclusions
Our repository of micro-CT scans represents a significant resource that can enhance our understanding ofXenopus’development and the associated morphological changes. The widespread utility of this amphibian species, coupled with the exceptional quality of our scans, which encompass a comprehensive series of developmental stages, opens up extensive opportunities for their broader research application. Moreover, these scans have the potential for use in virtual reality, 3D printing, and educational contexts, further expanding their value and impact.
Graphical abstract & lay summary
3D images of selected developmental stages ofX. laevisin a comparison (scale bar = 10 mm).
<fig id="ufig1" position="float" fig-type="figure" orientation="portrait"><graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="598452v1_ufig1" position="float" orientation="portrait"/></fig>Lay summary
X-ray tomography was used to examine the African clawed frog (Xenopus laevis). An extensive data set of specimens from tadpoles to adult frogs provides novel insights into the changes and developmental dynamics of selected structures, which opens avenues to an improved understanding of this crucial animal model.
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