Evaluation of a commercial Multiplex Real-Time PCR Assay for orthopoxvirus and monkeypox virus detection, and simultaneous subclade Ib identification

  1. Tony Wawina-Bokalanga
  2. Michael Haesler
  3. Prince Akil-Bandali
  4. Rilia Ola-Mpumbe
  5. Eddy Kinganda-Lusamaki
  6. Jean-Claude Makangara-Cigolo
  7. Elisabeth Pukuta-Simbu
  8. Fiston Cikaya-Kankolongo
  9. Nelson Mapenzi-Kashali
  10. Ange Ponga-Museme
  11. Tessa de Block
  12. Raphaël Lumembe-Numbi
  13. Adrienne Amuri-Aziza
  14. Gradi Luakanda
  15. Daan Jansen
  16. Koen Vercauteren
  17. Steve Ahuka-Mundeke
  18. Kai Schuchmann
  19. Placide Mbala-Kingebeni
  20. Jean-Jacques Muyembe-Tamfum
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Abstract

Several mpox outbreaks, caused by subclades Ia and Ib monkeypox virus (MPXV), are ongoing in the Democratic Republic of the Congo. The World Health Organization declared the ongoing mpox outbreak a Public Health Emergency of International Concern due to the geographic expansion of subclade Ib. We assessed the performance of a novel multiplex real-time PCR assay designed for MPXV detection and simultaneous subclade Ib identification. This assay demonstrates high accuracy and specificity, underscoring its use for the current mpox outbreak.

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