Enhanced recognition of topoisomerase 1 upon DNA binding by a subset of anti-topoisomerase 1 autoantibodies in systemic sclerosis
Abstract
Objectives
Systemic sclerosis (SSc) is a deleterious disease. Its clinical management is complicated by strong interpatient heterogeneity. Progressive organ fibrosis is linked to autoantibodies against topoisomerase 1 (TOP1). Here, we hypothesized that the molecular interaction between anti-TOP1 autoantibodies (ATAs) and TOP1 could be a relevant determinant of SSc pathogenesis. DNA binding by TOP1 might affect its interaction with ATAs. Therefore, we studied the effect of DNA binding by TOP1 on ATA recognition.
Methods
ATA monoclonal antibodies (ATA mAbs) were generated from patient-derived TOP1-reactive B cells. Reactivity of ATA mAbs and antibodies in patient plasma towards TOP1 and TOP1-DNA cleavage complexes (TOP1cc) was determined by ELISA and mass photometry. Immunostimulatory properties of ATA mAbs in complex with TOP1/TOP1cc were assessed by stimulation of monocytic THP-1 cells.
Results
DNA binding by TOP1 differentially affected the recognition of TOP1 by ATA mAbs. A subset of mAbs showed enhanced binding to TOP1cc, whereas others recognized TOP1 and TOP1cc to a similar extent. ATAs with enhanced TOP1cc recognition were observed in plasma of ATA+SSc patients and correlated with ATA levels and interstitial lung disease. These ‘TOP1cc-enhanced ATAs’ variably affected the enzymatic function of TOP1 and circulated predominantly as IgG1 and IgM. The interaction of ‘TOP1cc-enhanced ATA mAbs’ with TOP1cc strongly increased IL-8 production by THP-1 cells.
Conclusions
The differential recognition of TOP1 upon DNA binding by ATAs demonstrates heterogeneity in the ATA B cell response, possibly impacting on disease-relevant processes in severe SSc.
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