Test-retest reliability of multi-metabolite edited MRS at 3T using PRESS and sLASER

  1. Jessica Archibald
  2. Amy E. Bouchard
  3. Ralph Noeske
  4. Dikoma C. Shungu
  5. Mark Mikkelsen
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Abstract

Purpose

Spectral editing is the most common MRS approach for noninvasive in vivo measurement of low-concentration, strongly overlapped metabolites in the brain, such as γ-aminobutyric acid (GABA) and glutathione (GSH). Multi-metabolite editing methods, including HERMES and HERCULES, have recently been introduced, where multipleJ-coupled metabolites can be edited in a single acquisition without increasing total scan time. Yet little is known regarding the reliability of these methods. This study assessed the test-retest reliability of HERMES and HERCULES, where volume localization was achieved using either PRESS or sLASER.

Methods

Sixteen healthy adult volunteers were scanned twice in two separate sessions. Single-voxel edited MRS data were acquired in the medial parietal lobe using the following sequences: (1) HERMES-PRESS; (2) HERMES-sLASER; (3) HERCULES-PRESS; (4) HERCULES-sLASER. Spectra were processed and metabolites were quantified using the Osprey software. Data quality metrics and reliability statistics were estimated for all four acquisitions.

Results

HERMES-sLASER demonstrated lower within-subjects coefficients of variation (CVws) for GSH, glutamine (Gln), and glutamate (Glu) + Gln (Glx), suggesting improved reliability compared to HERMES-PRESS. However, GABA + co-edited macromolecules (GABA+) and Glu showed higher CVwsfor HERMES-sLASER. HERCULES-sLASER produced better reliability than HERCULES-PRESS for GABA+, GSH, Glu, Gln, Glx, aspartate (Asp), and lactate (Lac).N-acetylaspartate (NAA) andN-acetylaspartylglutamate (NAAG) showed higher CVwsfor HERCULES-sLASER. These findings suggest that sLASER may be more advantageous than PRESS for volume localization in simultaneous multi-metabolite editing.

Conclusion

Using sLASER yielded better test-retest reliability for most metabolites than using PRESS for volume localization for HERMES and HERCULES.

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