ascend: R package for analysis of single cell RNA-seq data
Abstract
Summary
ascend is an R package comprised of fast, streamlined analysis functions optimized to address the statistical challenges of single cell RNA-seq. The package incorporates novel and established methods to provide a flexible framework to perform filtering, quality control, normalization, dimension reduction, clustering, differential expression and a wide-range of plotting. ascend is designed to work with scRNA-seq data generated by any high-throughput platform, and includes functions to convert data objects between software packages.
Availability
The R package and associated vignettes are freely available at <ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="https://github.com/IMB-Computational-Genomics-Lab/ascend">https://github.com/IMB-Computational-Genomics-Lab/ascend</ext-link>.
Contact
<email>joseph.powell@uq.edu.au</email>
Supplementary information
An example dataset is available at ArrayExpress, accession number E-MTAB-6108
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