Multi-immersion open-top light-sheet microscope for high-throughput imaging of cleared tissues

  1. Adam K. Glaser
  2. Nicholas P. Reder
  3. Ye Chen
  4. Chengbo Yin
  5. Linpeng Wei
  6. Soyoung Kang
  7. Lindsey A. Barner
  8. Weisi Xie
  9. Erin F. McCarty
  10. Chenyi Mao
  11. Aaron R. Halpern
  12. Caleb R. Stoltzfus
  13. Jonathan S. Daniels
  14. Michael Y. Gerner
  15. Philip R. Nicovich
  16. Joshua C. Vaughan
  17. Lawrence D. True
  18. Jonathan T.C. Liu
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Abstract

Recent advances in optical clearing and light-sheet microscopy have provided unprecedented access to structural and molecular information from intact tissues. However, current light-sheet microscopes have imposed constraints on the size, shape, number of specimens, and compatibility with various clearing protocols. Here we present a multi-immersion open-top light-sheet microscope that enables simple mounting of multiple specimens processed with a variety of protocols, which will facilitate wider adoption by preclinical researchers and clinical laboratories.

One Sentence Summary

Glaseret al.describe a multi-immersion open-top light-sheet microscope that enables simple and high-throughput imaging of large numbers of preclinical and clinical specimens prepared with a variety of clearing protocols.

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