We present a genome assembly from a male specimen of Rhagio lineola (snipefly; Arthropoda; Insecta; Diptera; Rhagionidae). The genome sequence has a total length of 1,654.96 megabases. Most of the assembly (97.13%) is scaffolded into 5 chromosomal pseudomolecules, including the X sex chromosome. The mitochondrial genome has also been assembled, with a length of 16.64 kilobases.
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