Metagenomic Study on the Variable Influence of Soil Microbiomes on Oryza sativa Seedling Microbiome Across Different Cultivated Soils

Understanding the role of soil microbiome in shaping plant associated microbial communities is essential for enhancing plant health and productivity. The current study has been designed to investigate bacterial and fungal composition of root and leaf of rice plant grown under different soil types. By using the 16S and 18S rRNA amplicon sequencing, the study could identify distinct microbial profiles at the same time diversity analysis has revealed significant shifts in the root and leaf microbiomes, with root-associated communities displaying greater diversity in response to the soil variations. Notably, Xanthomonas was observed to be predominant in root samples grown in different soil conditions when compared to the controls, while the soil was observed to have a more diverse bacterial population. Fungal analysis has showed a high abundance of Aspergillus in both the soil and leaf samples, whereas Curvularia, Bipolaris , and Starmarella were abundant in root samples. These findings underscore the critical role of soil type in shaping the plant microbiome, influencing nutrient cycling, plant microbe interactions, and disease suppression. The result of the study provide insight into this phenomenon in rice by revealing the microbial differential distribution across the plant compartments and thereby highlighting the impact of soil on microbial assembly.