Molecular insights into Profilin1-dependent regulation of cellular phosphatidylinositol-(4,5)-bisphosphate

Phosphatidylinositol (4,5)-bisphosphate (PIP ₂ ), the most abundant cellular poly-phosphoinositide (PPI) class of phospholipid, is a central plasma membrane (PM)-associated signaling hub that controls many cellular processes. In this study, we demonstrate that either deletion of the gene encoding actin-binding protein profilin1 (Pfn1) or disruption of Pfn1-actin interaction leads to downregulation of PM PIP ₂ content in cells. This is also phenocopied when F-actin is depolymerized implying that Pfn1-dependent PIP ₂ alteration is related to its actin-regulatory function. Phospholipase C (PLC) activity is critical for Pfn1-deficient cells to exhibit the PIP ₂ -related phenotype. These findings, taken together with biochemical signatures of elevated PIP ₂ hydrolysis (higher baseline PM diacylglycerol-to PIP ₂ ratio and protein kinase C activity) exhibited by Pfn1-deficient cells, imply that PLC-mediated PIP ₂ hydrolysis plays a role in Pfn1-dependent regulation of PM PIP ₂ . Furthermore, we unexpectedly found that Pfn1 loss leads to dramatic alterations in several other important forms of lipids, revealing a previously unrecognized role of Pfn1 as a broad regulator of cellular lipid environment that extends beyond PPI control. In conclusion, our study establishes Pfn1 as an important regulator of cellular lipid homeostasis.