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A list by Prachee Avasthi Recommended Reading
See what researchers at Prachee Avasthi’s lab are reading to discover some interesting new work.
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A novel Fiji/ImageJ plugin for the rapid analysis of blebbing cells
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Emergence and maintenance of variable-length actin filaments in a limiting pool of building blocks
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Arp2/3 complex activity is necessary for mouse ESC differentiation, times formative pluripotency, and enables lineage specification
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IntAct: a non-disruptive internal tagging strategy to study actin isoform organization and function
This article's authorsAnnotation by Prachee Avasthi Recommended Reading
I’m certain I’ve highlighted this preprint before because it’s so interesting and useful but this is a new revision so check out the new analysis in figure 5. Tagging actin can affect its ability to polymerize, interact with its regulators, localize to the correct places, and perform its normal functions. This study identify an internal site to insert an epitope tag and tests those key features. As authors note, this is of particular interest to tag isoforms so their behaviors and functions can be teased apart.
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Mispatterned motile cilia beating causes flow blockage in the epileptic brain
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ChlamydomonasARMC2/PF27 is an obligate cargo adapter for IFT of radial spokes
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Molecular mechanism of Arp2/3 complex inhibition by Arpin
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Loss-of-function, gain-of-function and dominant-negative mutations have profoundly different effects on protein structure: implications for variant effect prediction
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The uptake of avermectins in Caenorhabditis elegans is dependent on Intra-Flagellar Transport and other protein trafficking pathways
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A kinesin-1 variant reveals motor-induced microtubule damage in cells
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