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A list by Prachee Avasthi Recommended Reading

See what researchers at Prachee Avasthi’s lab are reading to discover some interesting new work.

193 articles Last updated

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  • Conserved degronome features governing quality control associated proteolysis

    1. Bayan Mashahreh
    2. Shir Armony
    3. Kristoffer Enøe Johansson
    4. Alon Chappleboim
    5. Nir Friedman
    6. Richard G. Gardner
    7. Rasmus Hartmann-Petersen
    8. Kresten Lindorff-Larsen
    9. Tommer Ravid
    0 evaluations Published on Added on

    How interesting! A peptide screen to identify degrons with E3 ubiquitin ligase specificity in yeast followed by extraction of conserved properties identifies transmembrane domain containing peptides as likely degrons.

  • Integrating bulk and single cell RNA-seq refines transcriptomic profiles of specific C. elegans neurons

    1. Alec Barrett
    2. Erdem Varol
    3. Alexis Weinreb
    4. Seth R. Taylor
    5. Rebecca M. McWhirter
    6. Cyril Cros
    7. Manasa Basaravaju
    8. Abigail Poff
    9. John A. Tipps
    10. Maryam Majeed
    11. Berta Vidal
    12. Chen Wang
    13. Eviatar Yemini
    14. Emily A. Bayer
    15. HaoSheng Sun
    16. Oliver Hobert
    17. David M. Miller
    18. Marc Hammarlund
    0 evaluations Published on Added on

    Recognizing the utility of not using <strong>exclusively</strong> cutting edge methods for everything, this important study combines bulk RNAseq (high sensitivity due to capturing more low expression and noncoding RNAs) and single cell RNAseq (high specificity) for a more comprehensive transcriptomic picture.

  • Bio-hybrid micro-swimmers propelled by flagella isolated from C. reinhardtii†

    1. Raheel Ahmad
    2. Albert J Bae
    3. Yu-Jung Su
    4. Samira Goli Pozveh
    5. Eberhard Bodenschatz
    6. Alain Pumir
    7. Azam Gholami
    0 evaluations Published on Added on

    This is wild! Chlamydomonas flagella were isolated, demembranated, attached to polystyrene beads and reactivated to reconstitute flagellar movement. This yields some interesting insights through experimentation and simulation: Increased calcium concentration affects flagellar waveform and trajectory of bead movement and surprisingly, larger beads have increased rather than decreased velocity.

  • ARP2/3 complex associates with peroxisomes to participate in pexophagy in plants

    1. Jan Martinek
    2. Petra Cifrová
    3. Stanislav Vosolsobě
    4. Jana Krtková
    5. Lenka Sikorová
    6. Kateřina Malínská
    7. Zdeňka Mauerová
    8. Ian Leaves
    9. Imogen Sparkes
    10. Kateřina Schwarzerová
    0 evaluations Published on Added on

    Interesting role for ARP2/3 complex in peroxisome autophagy in plants!

  • Rapid Rerouting of Myosin Traffic at the T Cell Immunological Synapse

    1. Robert Mąka
    2. Natalia Plewa
    3. Urszula Cichoń
    4. Katarzyna Krysztofiak
    5. Jagoda J. Rokicka
    6. Ronald S. Rock
    0 evaluations Published on Added on

    This polarity of actin networks and myosin traffic is interesting in light of the parallels between cilium biogenesis and the immune synapse function.

  • Structural basis of actin filament assembly and aging

    1. Wout Oosterheert
    2. Björn U Klink
    3. Alexander Belyy
    4. Sabrina Pospich
    5. Stefan Raunser
    0 evaluations Published on Added on

    A 2.2 angstrom resolution structures of muscle actin filaments in ATP, ADP-Pi and ADP states. Many new insights here about the surprising stability of ADP actin, mechanism of ATP hydrolysis, cofilin binding and more.

  • Genome-wide functional analysis reveals key roles for kinesins in the mammalian and mosquito stages of the malaria parasite life cycle

    1. Mohammad Zeeshan
    2. Ravish Rashpa
    3. David J Ferguson
    4. Steven Abel
    5. Zeinab Chahine
    6. Declan Brady
    7. Sue Vaughan
    8. Carolyn A. Moores
    9. Karine G. Le Roch
    10. Mathieu Brochet
    11. Anthony A. Holder
    12. Rita Tewari
    5 evaluations Latest evaluation on Published on Added on

    Truly exquisite characterization of kinesins in the malaria parasite. Check out the spectacular expansion microscopy images in figure 7A!

  • The molecular organization of flat and curved caveolae indicates bendable structural units at the plasma membrane

    1. Claudia Matthaeus
    2. Kem A. Sochacki
    3. Andrea Dickey
    4. Dmytro Puchkov
    5. Volker Haucke
    6. Martin Lehmann
    7. Justin W. Taraska
    0 evaluations Published on Added on

    This is a beautiful characterization of caveolae. In addition to platinum replica EM of these plasma membrane invaginations in various cell types, this work includes molecular identification of proteins associated with caveolae of different curvatures by STED-CLEM (very high-resolution correlative light electron microscopy).

  • Early Endosome Capture Proteomics and its Application to Amyloid Precursor Protein Intramembrane Processing by β and γ-Secretases

    1. Hankum Park
    2. Qing Yu
    3. Frances V. Hundley
    4. Katherine Overmyer
    5. Lia Serrano
    6. Joao A. Paulo
    7. Julia C. Paoli
    8. Sharan Swarup
    9. Joshua J. Coon
    10. Steven P. Gygi
    11. J. Wade Harper
    0 evaluations Published on Added on

    Whoa! Successful ID of early endosomal cargoes via pull-down of an early endosomal marker followed by proteomics.

  • Systematic analysis of cilia characteristics and Hedgehog signaling in five immortal cell lines

    1. Arianna Gómez
    2. Julie Craft Van De Weghe
    3. Malaney Finn
    4. Dan Doherty
    0 evaluations Published on Added on

    I love this kind of study that challenges our assumptions of what cells and models we should use. This is an important comparative characterization of cilium-based hedgehog signaling across a variety of cultured cell types.

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