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A list by Prachee Avasthi Recommended Reading
See what researchers at Prachee Avasthi’s lab are reading to discover some interesting new work.
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Flagellar energy costs across the Tree of Life
This article's authorsAnnotation by Prachee Avasthi Recommended Reading
Incredibly cool analysis of energetic costs of flagellar construction and operation in bacteria, archaea, and eukaryotes and proposes that only larger cells can afford the eukaryotic flagellum. Super interesting example of micromonas (tiny algae closer to prokaryote size) that have a minimal eukaryotic flagellum.
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A chemical biology toolbox to investigate in-cell target engagement and specificity of PRMT5-inhibitors
This article's authorsAnnotation by Prachee Avasthi Recommended Reading
Nice method that reads out small molecule interaction with its cellular target in live cells using bioluminescence resonance energy transfer. The target of interest is fused with a luciferase and the small molecule is conjugated to a fluorophore.
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Verification of extracellular vesicle-mediated functional mRNA delivery via RNA editing
This article's authorsAnnotation by Prachee Avasthi Recommended Reading
Really clever method of detecting bona fide mRNA delivery from extracellular vesicles into recipient cells!
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Puromycin is incorporated into regenerating flagella of Chlamydomonas reinhardtii as an indicator of nascent flagellar proteins
This article's authorsAnnotation by Prachee Avasthi Recommended Reading
Interesting paper recapitulating the finding that newly synthesized proteins are incorporated at distal tips into Chlamy cilia using puromycin labelling. This is during post-pH shock mediated reassembly. Previously @wallaceUCSF found the distal incorporation using labelled tubulin in dikaryons.
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Multiple Ciliary Localization Signals Control INPP5E Ciliary Targeting
This article's authorsAnnotation by Prachee Avasthi Recommended Reading
Unprecedented complexity and redundancy in ciliary targeting signals of the ciliary phosphoinositide phosphatase, INPP5E.
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Genomic instability caused by Arp2/3 complex inactivation results in micronucleus biogenesis and cellular senescence
This article's authorsAnnotation by Prachee Avasthi Recommended Reading
Whoa, mitotic spindle and chromosome segregation defects during mitosis in an ARPC2 conditional knockout to disrupt the Arp2/3 complex
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The architecture of the actin network can balance the pushing forces produced by growing microtubules
This article's authorsAnnotation by Prachee Avasthi Recommended Reading
Another great paper from the CytomorphoLab using a cell-free system to determine the interplay of actin networks and microtubule forces in centrosome positioning.
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Amphibian mucus triggers a developmental transition in the frog-killing chytrid fungus
This article's authorsAnnotation by Prachee Avasthi Recommended Reading
Awesome study from the @FritzLaylin group on the calcium-dependent encystation of chytrid fungi in response to mucin/mucous.
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Comparative Phenotyping of Two Commonly Used Chlamydomonas reinhardtii Background Strains: CC-1690 (21gr) and CC-5325 (the CLiP Mutant Library Background)
This article's authorsAnnotation by Prachee Avasthi Recommended Reading
Important characterization of two of our commonly used wild type Chlamy strains.
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Actin-microtubule dynamic composite forms responsive active matter with memory
This article's authors