• Flagellar energy costs across the Tree of Life

    1. Paul E Schavemaker
    2. Michael Lynch
    This article has 4 evaluations Latest evaluation on Published on Added on

    Annotation by Prachee Avasthi Recommended Reading

    Incredibly cool analysis of energetic costs of flagellar construction and operation in bacteria, archaea, and eukaryotes and proposes that only larger cells can afford the eukaryotic flagellum. Super interesting example of micromonas (tiny algae closer to prokaryote size) that have a minimal eukaryotic flagellum.

  • A chemical biology toolbox to investigate in-cell target engagement and specificity of PRMT5-inhibitors

    1. Elisabeth M. Rothweiler
    2. Jakub Stefaniak
    3. Jennifer A. Ward
    4. Catherine Rogers
    5. Esra Balikci
    6. Kilian V. M. Huber
    This article has 0 evaluations Published on Added on

    Annotation by Prachee Avasthi Recommended Reading

    Nice method that reads out small molecule interaction with its cellular target in live cells using bioluminescence resonance energy transfer. The target of interest is fused with a luciferase and the small molecule is conjugated to a fluorophore.

  • Puromycin is incorporated into regenerating flagella of Chlamydomonas reinhardtii as an indicator of nascent flagellar proteins

    1. Tomohiro Kubo
    2. Natsumi Kanou
    3. Toshiyuki Oda
    This article has 0 evaluations Published on Added on

    Annotation by Prachee Avasthi Recommended Reading

    Interesting paper recapitulating the finding that newly synthesized proteins are incorporated at distal tips into Chlamy cilia using puromycin labelling. This is during post-pH shock mediated reassembly. Previously @wallaceUCSF found the distal incorporation using labelled tubulin in dikaryons.

  • Multiple Ciliary Localization Signals Control INPP5E Ciliary Targeting

    1. Dario Cilleros-Rodriguez
    2. Raquel Martin-Morales
    3. Pablo Barbeito
    4. Abhijit Deb Roy
    5. Abdelhalim Loukil
    6. Belen Sierra-Rodero
    7. Gonzalo Herranz
    8. Olatz Pampliega
    9. Modesto Redrejo-Rodriguez
    10. Sarah C. Goetz
    11. Manuel Izquierdo
    12. Takanari Inoue
    13. Francesc R. Garcia-Gonzalo
    This article has 4 evaluations Latest evaluation on Published on Added on

    Annotation by Prachee Avasthi Recommended Reading

    Unprecedented complexity and redundancy in ciliary targeting signals of the ciliary phosphoinositide phosphatase, INPP5E.

  • The architecture of the actin network can balance the pushing forces produced by growing microtubules

    1. Shohei Yamamoto
    2. Jérémie Gaillard
    3. Benoit Vianay
    4. Christophe Guerin
    5. Magali Orhant-Prioux
    6. Laurent Blanchoin
    7. Manuel Théry
    This article has 4 evaluations Latest evaluation on Published on Added on

    Annotation by Prachee Avasthi Recommended Reading

    Another great paper from the CytomorphoLab using a cell-free system to determine the interplay of actin networks and microtubule forces in centrosome positioning.

  • Amphibian mucus triggers a developmental transition in the frog-killing chytrid fungus

    1. Kristyn A Robinson
    2. Sarah M Prostak
    3. Evan H Campbell Grant
    4. Lillian K Fritz-Laylin
    This article has 0 evaluations Published on Added on

    Annotation by Prachee Avasthi Recommended Reading

    Awesome study from the @FritzLaylin group on the calcium-dependent encystation of chytrid fungi in response to mucin/mucous.

  • Comparative Phenotyping of Two Commonly Used Chlamydomonas reinhardtii Background Strains: CC-1690 (21gr) and CC-5325 (the CLiP Mutant Library Background)

    1. Ningning Zhang
    2. Leila Pazouki
    3. Huong Nguyen
    4. Sigrid Jacobshagen
    5. Ming Xia
    6. Anastasiya Klebanovych
    7. Kirk J. Czymmek
    8. Ru Zhang
    This article has 0 evaluations Published on Added on

    Annotation by Prachee Avasthi Recommended Reading

    Important characterization of two of our commonly used wild type Chlamy strains.