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A list by Prachee Avasthi Recommended Reading

See what researchers at Prachee Avasthi’s lab are reading to discover some interesting new work.

193 articles Last updated

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  • Granger-causal inference of the lamellipodial actin regulator hierarchy by live cell imaging without perturbation

    1. Jungsik Noh
    2. Tadamoto Isogai
    3. Joseph Chi
    4. Kushal Bhatt
    5. Gaudenz Danuser
    0 evaluations Published on Added on

    Super interesting study on inferring causal relationships between molecular pathways and cell behavior, something I’m very interested in given molecular detail isn’t accessible for so many cells/species.

  • Targeted protein degradation using degradFP in Trypanosoma brucei

    1. Midori Ishii
    2. Bungo Akiyoshi
    0 evaluations Published on Added on

    Nice! Targeted degradation of GFP tagged proteins in Trypanosomes. They applied degradFP, which fuses a GFP nanobody with a protein that recruits an E3 ubiquitin ligase, causing ubiquitination and degradation of the GFP-tagged target.

  • TTBK2 controls cilium stability through actin and the centrosomal compartment

    1. Abraham Nguyen
    2. Sarah C. Goetz
    4 evaluations Latest evaluation on Published on Added on

    Some interesting interactions here consistent with actin’s role in restricting mammalian ciliogenesis: decreased ciliation and increased breakage caused by inducible disruption of Tau Tubulin Kinase 2 is partially rescued by inhibitors of actin polymerization and myosin VI.

  • Improved methods for bulk cultivation and fixation of Loxodes ciliates for fluorescence microscopy

    1. Brandon Kwee Boon Seah
    2. Christiane Emmerich
    3. Aditi Singh
    4. Estienne Swart
    0 evaluations Published on Added on

    Quite impressive morphology preservation and immunofluorescence results with a new fixative containing zinc sulfate, formaldehyde, acetic acid, & ethanol for Loxodes (a ciliate genus)

  • Cell walls are dynamically O-acetylated in the green seaweed, Ulva compressa

    1. John H. Bothwell
    2. Alexander J. Goodridge
    3. Marie Rapin
    4. Patrick J. Brennan
    5. Alexandra Traslaviña López
    6. Akanksha Agrawal
    7. Stephen C. Fry
    8. Georgia Campbell
    9. Jonas Blomme
    0 evaluations Published on Added on

    An interesting paper identifying stress-induced modification (O-acetylation) in the cell wall of the green seaweed, Ulva compressa. It contains a protocol for chlorophyte cell wall fractionation.

  • Signal Peptide Efficiency: from High-throughput Data to Prediction and Explanation

    1. Stefano Grasso
    2. Valentina Dabene
    3. Margriet M.W.B. Hendriks
    4. Priscilla Zwartjens
    5. René Pellaux
    6. Martin Held
    7. Sven Panke
    8. Jan Maarten van Dijl
    9. Andreas Meyer
    10. Tjeerd van Rij
    0 evaluations Published on Added on

    Whoa nice, a systematic analyses of thousands of signal peptide sequences and effects on properties affecting secretion

  • ERK pathway activation inhibits ciliogenesis and causes defects in motor behavior, ciliary gating, and cytoskeletal rearrangement

    1. Larissa L Dougherty
    2. Soumita Dutta
    3. Prachee Avasthi
    9 evaluations Latest evaluation on Published on Added on

    Again I get to highlight one of our new preprints! This one tackles the intricate interplay between cell division and ciliogenesis. Using genetics, biochemistry, four different imaging modalities, and two organisms, we identified cytoskeletal, motor, and trafficking defects causing impaired cilium formation when activating a major cell proliferation and cancer pathway.

  • Blind spots on western blots: a meta-research study highlighting opportunities to improve figures and methods reporting

    1. Cristina Kroon
    2. Larissa Breuer
    3. Lydia Jones
    4. Jeehye An
    5. Ayça Akan
    6. Elkhansa Ahmed Mohamed Ali
    7. Felix Busch
    8. Marinus Fislage
    9. Biswajit Ghosh
    10. Max Hellrigel-Holderbaum
    11. Vartan Kazezian
    12. Alina Koppold
    13. Cesar Alberto Moreira Restrepo
    14. Nico Riedel
    15. Lea Scherschinski
    16. Fernando Raúl Urrutia Gonzalez
    17. Tracey Weissgerber
    0 evaluations Published on Added on

    There may not be a technique as over-interpreted relative to the information that it can provide as a western blot but this is made worse by ignoring best practices and my favorite, using a single protein as an unvalidated loading control. This analysis of westerns from the literature highlights some better display and reporting practices

  • Liquid-like VASP condensates drive actin polymerization and dynamic bundling

    1. Kristin Graham
    2. Aravind Chandrasekaran
    3. Liping Wang
    4. Aly Ladak
    5. Eileen M. Lafer
    6. Padmini Rangamani
    7. Jeanne C. Stachowiak
    0 evaluations Published on Added on

    I have no idea whether this actually happens in a cell but what an interesting model! I think I audibly gasped at figure 3A.

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