• Ultrastructure Expansion Microscopy reveals the nanoscale cellular architecture of budding and fission yeast

    1. Kerstin Hinterndorfer
    2. Marine. H. Laporte
    3. Felix Mikus
    4. Lucas Tafur Petrozzi
    5. Clélia Bourgoint
    6. Manoel Prouteau
    7. Gautam Dey
    8. Robbie Loewith
    9. Paul Guichard
    10. Virginie Hamel
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    Annotation by Prachee Avasthi Recommended Reading

    Spectacular expansion microscopy in yeast! I went straight for the cell wall digestion protocol so we can try it in a number of different algae

  • Condensation of the fusion focus by the intrinsically disordered region of the formin Fus1 is essential for cell-cell fusion

    1. Ingrid Billault-Chaumartin
    2. Olivia Muriel
    3. Laetitia Michon
    4. Sophie G Martin
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    Annotation by Prachee Avasthi Recommended Reading

    This is so cool! The intrinsically disordered region in a pombe formin is needed to generate the foci needed for actin mediated cell fusion. Both replacement and rescue generate the expected behaviors associated with condensation state. Lots of interesting papers recently on condensates mediating behaviors of actin regulators and polymerization!

  • Protrusion growth driven by myosin-generated force

    1. Gillian N. Fitz
    2. Meredith L. Weck
    3. Caroline Bodnya
    4. Olivia L. Perkins
    5. Matthew J. Tyska
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    Annotation by Prachee Avasthi Recommended Reading

    Interesting study showing that membrane-anchored myosin can generate the force necessary for membrane protrusion beyond actin polymerization based mechanisms

  • Centriole growth is not limited by a finite pool of components, but is limited by the Cdk1/Cyclin-dependent phosphorylation of Ana2/STIL

    1. Thomas L. Steinacker
    2. Siu-Shing Wong
    3. Zsofia A. Novak
    4. Saroj Saurya
    5. Lisa Gartenmann
    6. Eline J.H. van Houtum
    7. Judith R. Sayers
    8. B. Christoffer Lagerholm
    9. Jordan W. Raff
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    Annotation by Prachee Avasthi Recommended Reading

    Limiting component models in organelle size control are difficult to pin down as the limiting components themselves are hard to identify. This study identifies an alternate kinase-dependent model for centriole size control.

  • Lithium-induced ciliary lengthening sparks Arp2/3 complex-dependent endocytosis

    1. Brae M Bigge
    2. Prachee Avasthi
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    Annotation by Prachee Avasthi Recommended Reading

    This week, I get to highlight our own preprint proposing a putative mechanism of lithium-mediated cilium extension through stimulating endocytosis to supply membrane to the cilium from the plasma membrane. We proposed this rapid route for ciliary material in our last preprint

  • Combined nanometric and phylogenetic analysis of unique endocytic compartments in Giardia lamblia sheds light on the evolution of endocytosis in Fornicata

    1. Rui Santos
    2. Ásgeir Ástvaldsson
    3. Shweta V. Pipaliya
    4. Jon Paulin Zumthor
    5. Joel B. Dacks
    6. Staffan Svärd
    7. Adrian B. Hehl
    8. Carmen Faso
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    Annotation by Prachee Avasthi Recommended Reading

    Incredibly interesting study of endocytic adaptation in an obligate parasite in which a structural analog of clathrin light chain is predicted/identified.

  • Kinesin-1 transports morphologically distinct intracellular virions during vaccinia infection

    1. Amadeus Xu
    2. Angika Basant
    3. Sibylle Schleich
    4. Timothy P Newsome
    5. Michael Way
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    Annotation by Prachee Avasthi Recommended Reading

    Do yourself a favor and check out this exquisite characterization of vaccinia viron transport towards microtubule plus ends. In-cell deletion and microscopy, in vitro reconstitution, quantification of motor complexes associated with virons and viron speed … really beautiful work!