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A list by Prachee Avasthi Recommended Reading

See what researchers at Prachee Avasthi’s lab are reading to discover some interesting new work.

193 articles Last updated

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  • The actin assembly requirements of the formin Fus1 to build the fusion focus

    1. Ingrid Billault-Chaumartin
    2. Laetitia Michon
    3. Caitlin A Anderson
    4. Sarah E Yde
    5. Cristian Suarez
    6. Justyna Iwaszkiewicz
    7. Vincent Zoete
    8. David R Kovar
    9. Sophie G Martin
    3 evaluations Latest evaluation on Published on Added on

    This is a great demonstration (using chimeras of formin FH2 and FH1 domains from a diverse range of formins) that tuning the actin nucleation and elongation rates of formins affects actin architecture. This can help explain how various cell types can generate unique actin structures optimized for a particular function as in this case for Fus1’s role in assembling the structure required for fusion of fission yeast cells during mating.

  • Vimentin intermediate filaments organize organellar architecture in response to ER stress

    1. Tom Cremer
    2. Lenard M. Voortman
    3. Erik Bos
    4. Daphne M. van Elsland
    5. Laurens R. ter Haar
    6. Roman I. Koning
    7. Ilana Berlin
    8. Jacques Neefjes
    0 evaluations Published on Added on

    Interesting function of intermediate filaments though an ER embedded protein: this protein, a ubiquitin ligase, links the perinuclear ER with vimentin-based intermediate filaments for ER and stress-dependent endosome positioning. The work was done in U2OS cells.

  • High-resolution secretory timeline from vesicle formation at the Golgi to fusion at the plasma membrane in S. cerevisiae

    1. Robert M. Gingras
    2. Abigail M. Sulpizio
    3. Joelle Park
    4. Anthony Bretscher
    5 evaluations Latest evaluation on Published on Added on

    This is a must-read spatio-temporal characterization of secretory pathway events during yeast exocytosis. This will undoubtedly impact our thinking about secretory traffic during processes like encystment and ciliogenesis across a range of unicellular eukaryotes.

  • Imaging tools generated by CRISPR/Cas9 tagging reveal cytokinetic diversity in mammalian cells

    1. Mathieu C. Husser
    2. Imge Ozugergin
    3. Tiziana Resta
    4. Vincent J. J. Martin
    5. Alisa J. Piekny
    0 evaluations Published on Added on

    This paper is a good reminder of the heterogeneity in phenotypes that can be observed when visualizing molecular players at physiological levels.

  • Identifying the genes impacted by cell proliferation in proteomics and transcriptomics studies

    1. Marie Locard-Paulet
    2. Oana Palasca
    3. Lars Juhl Jensen
    0 evaluations Published on Added on

    Interesting framing…identifying genes that are robustly correlated with cell proliferation not to find novel mechanisms of division but to eliminate confounding contributions of cell proliferation in cellular processes analyzed by large data sets.

  • Alterations to the broad-spectrum formin inhibitor SMIFH2 improve potency

    1. Marina Orman
    2. Maya Landis
    3. Aisha Oza
    4. Deepika Nambiar
    5. Joana Gjeci
    6. Kristen Song
    7. Vivian Huang
    8. Amanda Klestzick
    9. Carla Hachicho
    10. Su Qing Liu
    11. Judith M. Kamm
    12. Francesca Bartolini
    13. Jean J. Vadakkan
    14. Christian M. Rojas
    15. Christina L. Vizcarra
    0 evaluations Published on Added on

    Thanks to @cdmacquarrie for alerting me to this one! Synthesis of SMIFH2 derivatives identifies more potent versions (5-fold decrease in IC50) that are able to inhibit all human formins. This increased potency may reduce off target inhibition of myosins at higher concentrations. Myosins aren’t directly tested in this study.

  • Transient accumulation and bidirectional movement of KIF13B in primary cilia

    1. Alice Dupont Juhl
    2. Zeinab Anvarian
    3. Stefanie Kuhns
    4. Julia Berges
    5. Jens S. Andersen
    6. Daniel Wüstner
    7. Lotte B. Pedersen
    0 evaluations Published on Added on

    Worm cilia are perhaps not as unique as we thought. A kinesin-3 accumulates in a subset of cilia in human cultured cells and undergoes bursts of bidirectional motor domain dependent movement.

  • The dynamics of protein localisation to restricted zones within Drosophila mechanosensory cilia

    1. Wangchu Xiang
    2. Petra zur Lage
    3. Fay G. Newton
    4. Guiyun Qiu
    5. Andrew P. Jarman
    0 evaluations Published on Added on

    Very interesting study tackling the sub-compartmentalization of cilia. In fly chorodonal neurons, cilia have differences in outer and inner dynein arm complexes along the apical-distal axis of the axoneme. This paper highlights different modes of establishing this subcompartmentalization whether by restriction to proximal regions (ODAs) or by uniform localization and maturation to final proximal restriction (IDAs). Access to the axonemal interior, binding affinities via docking complexes, tubulin modification and pruning of IDA localization by retrograde IFT-mediated removal are suggested as possible factors.

  • Moving Yeasts: Resolving the Mystery

    1. Gulab Puri
    2. Sulbha Chaudhari
    3. Areeb Inamdar
    4. Manish Kohli
    5. Vishal Sangawe
    6. Chandrakant Jadhav
    7. Lakshman Teja
    8. Nitin Adhapure
    0 evaluations Published on Added on
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